Journal: Scientific Reports

Article Title: Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines

doi: 10.1038/s41598-021-98869-x

Figure Lengend Snippet: Optimization of the reverse transcription conditions. The graph shows Cq values from performing qPCR measurements of RNA dilution series comparing the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (red lines) and the High-Capacity cDNA Reverse Transcription Kit (blue lines). Three technical replicates for both kits at each concentration point are marked as hollow circles. Least squares linear regression was performed to the average of the technical replicates in the range of RNA amount from 100 to 800 ng per reaction. Individual graphs were created with OriginPro 2018 (OriginLab Corp.) and the figure was assembled using CorelDRAW Graphics Suite 2020 (Corel Corporation).

Article Snippet: Graphs were created with OriginPro 2018 (OriginLab Corp.) (URL: https://www.originlab.com/2018 ).

Techniques: Quantitative RT-PCR, Concentration Assay

Journal: Scientific Reports

Article Title: Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines

doi: 10.1038/s41598-021-98869-x

Figure Lengend Snippet: Cq values of twelve candidate reference genes in human cancer (red) and normal (blue) cell lines. Each dot represents the average Cq value of three biological replicates for each cell line. Candidate reference genes are arranged according to the comprehensive ranking in a decreasing order of expression stability. The boxes represent the data between the 25th and 75th percentile, while whiskers show the data range from the 10th to the 90th percentile. The minimum and maximum values are depicted as ‘x’. The average values are represented as squares and the median values are illustrated as lines. This graph was created with OriginPro 2018 (OriginLab Corp.).

Article Snippet: Graphs were created with OriginPro 2018 (OriginLab Corp.) (URL: https://www.originlab.com/2018 ).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines

doi: 10.1038/s41598-021-98869-x

Figure Lengend Snippet: Relative expression of the candidate reference genes upon serum starvation. Relative expression values of serum starved cell lines can be compared to the average relative expression of the non-treated biological replicates selected as control and set to 1, which is represented by a dotted line. Error bars show standard deviation of three biological replicates (n = 3) for each cell line. The number of asterisks indicate increasing possibility that gene expression remains constant upon serum starvation. *p > 0.8, **p > 0.95 as calculated by the CFX Maestro software. This graph was created with OriginPro 2018 (OriginLab Corp.).

Article Snippet: Graphs were created with OriginPro 2018 (OriginLab Corp.) (URL: https://www.originlab.com/2018 ).

Techniques: Expressing, Standard Deviation, Software